产品简介
NUCLEODUR C18 Gravity液相色谱柱
C18Gravity：PH稳定应用范围宽达1.0—11.0
※ 满足您对方法开发
※ 端PH应用的需求
※ 和复杂样品的分离
Gravity是在NUCLEODUR纯硅胶基础上，采用 18%高碳载量和多步交联键合技术。适合强碱性样品的分析，而且能在PH=1.0—11.0范围内稳定使用,合美国药典USP L1，常卖货号760101.46C18 Gravity 4.6X250mm 5um
性能：
主要特点：
适用于液相色谱/质谱和液相色谱法在PH（PH值1 - 11）
的基失活
方法开发的理想
技术特点：
十八烷基改性高密度、多封端
孔径110A；粒径1.8μm，3 m和5 mμ
10，7，12和16μm粒子的制备要
碳含量18%
推荐应用：
整体复杂的分析分离
复合类分离包括，如镇痛药、药、抑郁药；除草剂；植物；免疫抑制剂
Standard program* of columns packed with NUCLEODUR? C18 Gravity | ||||||
Column system | 粒径: | 1.8 μm | 3 μm | 5 μm | 10 μm | |
分析柱 | EC columns (standard system, 2–4.6 mm) |
| + | + | + | + |
制备 | VarioPrep columns (VP, ≥ 8 mm) |
| + | + |
液相色谱分析法
订货信息：
Length -> | 30 mm | 50 mm | 75 mm | 100 mm | 125 mm | 150 mm | 250 mm |
NUCLEODUR? C18 Gravity, 1.8 μm | |||||||
2 mm ID | 760078.20 | 760079.20 | 760071.20 | 760076.20 | 760075.20 | ||
3 mm ID | 760078.30 | 760079.30 | 760076.30 | ||||
4 mm ID | 760078.40 | 760079.40 | 760076.40 | ||||
4.6 mm ID | 760078.46 | 760079.46 | 760076.46 | ||||
EC guard columns* | 4 x 2 mm | 761901.20 | 4 x 3 mm | 761901.30 | |||
NUCLEODUR? C18 Gravity, 3 μm | |||||||
2 mm ID | 760080.20 | 760084.20 | 760081.20 | 760083.20 | 760082.20 | ||
3 mm ID | 760080.30 | 760084.30 | 760081.30 | 760083.30 | 760082.30 | ||
4 mm ID | 760080.40 | 760084.40 | 760081.40 | 760083.40 | 760082.40 | ||
4.6 mm ID | 760080.46 | 760086.46 | 760084.46 | 760081.46 | 760083.46 | 760082.46 | |
EC guard columns* | 4 x 2 mm | 761902.20 | 4 x 3 mm | 761902.30 | |||
NUCLEODUR? C18 Gravity, 5 μm | |||||||
2 mm ID | 760102.20 | 760104.20 | 760100.20 | 760103.20 | 760101.20 | ||
3 mm ID | 760102.30 | 760104.30 | 760100.30 | 760103.30 | 760101.30 | ||
4 mm ID | 760102.40 | 760104.40 | 760100.40 | 760103.40 | 760101.40 | ||
4.6 mm ID | 760102.46 | 760106.46 | 760104.46 | 760100.46 | 760103.46 | 760101.46 | |
EC guard columns* | 4 x 2 mm | 761903.20 | 4 x 3 mm | 761903.30 |
Guard columns for EC columns | EC columns with ID | Use guard | |||||
2 mm | 3 mm | 4 mm | 4.6 mm | ||||
* | Column Protection System | EC guard column | 4/2 | 4/3 | 4/3 | 4/3 | REF 718966 |
VarioPrep 柱用在液相制备
Length | 10 mm ID | 21 mm ID | 32 mm ID | 40 mm ID |
NUCLEODUR? C18 Gravity, 5 μm | ||||
50 mm | 762103.100 | 762103.210 | ||
125 mm | 762109.100 | 762109.210 | ||
150 mm | 762100.400 | |||
250 mm | 762113.100 | 762113.210 | 762113.320 | 762113.400 |
NUCLEODUR? C18 Gravity, 10 μm | ||||
250 mm | 762250.210 | 762250.400 | ||
VP guard columns with NUCLEODUR? C18 Gravity | ||||
8 mm ID | 16 mm ID | 32 mm ID | ||
10 mm* | 762160.80 | 762160.160 | ||
15 mm* | 762163.320 |
* VP guard column | Size | Pack of | for VP column | required holder | REF |
VP 10/8 | 10 x 8 mm ID | 2 | 10 mm ID | 8 mm | 718251 |
VP 10/16 | 10 x 16 mm ID | 2 | 21 mm ID | 16 mm | 718256 |
VP 15/32 | 15 x 32 mm ID | 1 | 32–40 mm ID | 32 mm | 718253 |
对照品舒巴坦钠的标定
使用NUCLEODUR C18 Gravity和对照柱X按照《中国药典》方法检测进行标定
色谱柱：NUCLEODUR C18 Gravity 250×4.6mm 5um 对照柱X 250×4.6mm 5um
流动相：0.005 mol/L 四丁基氢氧化铵（PH=5.0）:乙腈=75:25
温 度：35℃
检测器：UV 230nm
结果:
舒巴坦钠理论塔板数 | 舒巴坦钠 | 重现性 | |
C18 Gravity | 7398 | 4.13 | 0.71 |
对照柱X | 6317 | 4.00 | 0.15 |
应用：
1、 万古霉素
Substances: | vancomycin | |
Column: | EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm | |
Phase: | NUCLEODUR? C18 Gravity | |
REF number: | 760101.46 | |
Sample pretreatment: | Sample concentration: 2 mg/mL in eluent A | |
Conditions: | Eluent A: 920 mL stock solution + 10 mL THF + 70 mL ACN | |
Detection: | UV, 280 nm | |
Author(-s): | HPLC department | |
Source: | MACHEREY-NAGEL, Germany 2010 | |
Keywords: | antibiotics; pharmacopeia; Pharm. Eu.; asymmetry factor | |
Legend: | 1 - vancomycin | |
Note: | Vancomycin is determined with an asymmetry factor of 1.12 (Pharm. Eu< 1.60) |
Substances: | acetylsalicylic acid; sulindac; piroxicam; suprofen; tolmetin; naproxen; diflunisal; fenoprofen; flurbiprofen; niflumic acid; indomethacin; ibuprofen | |
Column: | EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm | |
Phase: | NUCLEODUR? C18 Gravity | |
MN catalog number: | 760101.46 | |
Conditions: | Mobile phase: acetonitrile / 20 mM KH2PO4, pH 2.5, 45:55 (v/v) | |
Detection: | UV, 230 nm | |
Author(-s): | HPLC department | |
Source: | MACHEREY-NAGEL, Germany 2008 | |
Keywords: | antiinflamatoray drugs; drugs; NSAID | |
Legend: | 1 - acetylsalicylic acid |
Substances: | coumafuryl; warfarin; pindone; coumatetralyl; coumachlor; diphacinone; chlorphacinone; bromadiolone i; difenacoum; bromadiolone ii; flocoumafen i; flocumafen ii; brodifacoum; difethialone; pyranocoumarin | |
Column: | EC 250/4.6 NUCLEODUR? C18 Gravity, 5 μm | |
Phase: | NUCLEODUR? C18 Gravity | |
REF number: | 760101.46 | |
Conditions: | Buffer: | |
Detection: | A) DAD UV, 308 nm (285 nm, 260 nm) | |
Author(-s): | Freidhof, K. | |
Source: | LandesuntersuchungsamtRheinland-Pfalz, Koblenz; Germany | |
Keywords: | anti-coagulant drugs; drugs | |
Legend: | Peaks: |
