QE65000 是美国海洋光学公司研制的一款高的科研级光谱仪——其光谱分辨率为 ~0.14-7.7 nm FWHM；
高灵敏度——22 electroncs/count all ٨; 26 photons/count at 250 nm；
低噪声——其信噪比可达1000:1 (at full signal)，由于检测器采用TE制冷，其温度可达到-15度，
由此大大的降低了噪声水平，可测量微弱光——譬如荧光测量；
光谱范围——200-1100nm，适宜多用途测量（例如：测量荧光，DNA序列，天文学以及拉曼光谱）。
该光谱仪采用了2维面阵检测器，所以其感光敏感度明显优于普通线阵检测器光谱仪。
更详细的技术参数请见下面介绍：
Onboard Programming
The QE65000 also has an onboard programmable micro-controller for controlling the spectrometer and accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.
Optical Bench Options
At Ocean Optics, we understand that your work is unique and that you want a spectrometer to fit your specific needs. With the QE65000 Spectrometer, you can configure the bench for your application. Choose QE65000 Optical Bench Options such as entrance aperture size, detector accessories, filters, gratings and more. Our application scientists will help you determine which configuration will best work for you.
Variety of Filter Options Available
We have a number of variable bandpass order-sorting filter options available to vary the starting wavelength at 50 nm intervals to fit your particular application. These OFLVs are for use with the HC-1 grating only and consist of the following:
Triggering Options
The QE65000 is available with external triggering that helps you easily synchronize data acquisition with external events. Check out the documentation (PDF format):
Our QE65000 Manual provides you with instructions for setting up, calibrating and performing experiments with your QE65000 spectrometer.
Specifications
高灵敏度——22 electroncs/count all ٨; 26 photons/count at 250 nm；
低噪声——其信噪比可达1000:1 (at full signal)，由于检测器采用TE制冷，其温度可达到-15度，
由此大大的降低了噪声水平，可测量微弱光——譬如荧光测量；
光谱范围——200-1100nm，适宜多用途测量（例如：测量荧光，DNA序列，天文学以及拉曼光谱）。
该光谱仪采用了2维面阵检测器，所以其感光敏感度明显优于普通线阵检测器光谱仪。
更详细的技术参数请见下面介绍：
| QE65000 Scientific-grade Spectrometer The QE65000 Scientific-grade Spectrometer is a novel combination of detector, optical bench and electronics technologies that provides users with a remarkably sensitive system for demanding low-light level applications such as fluorescence, DNA sequencing, astronomy and Raman spectroscopy. The QE65000 can achieve up to 90% quantum efficiency with high signal-to-noise and rapid signal processing speed. New! Optical Bench Enhancements The great performance of our QE65000 Spectrometer has been enhanced to provide even more value: lower stray light, improved efficiency in the UV and Shortwave NIR, and better unit-to-unit reproducibility. For full UV-Shortwave NIR coverage, you'll want to configure your QE65000 with our HC-1 composite grating. Quantum Efficiency to 90% The QE65000 is the most sensitive spectrometer we have ever developed. The Hamamatsu FFT-CCD detector used in the QE65000 provides 90% quantum efficiency (defined as how efficiently a photon is converted to a photo-electron). Most of our other detectors are linear CCDs but with this “2D” area detector, we can bin (or sum) a vertical row of pixels, which offers significant improvement in the signal-to-noise ratio (>1000:1) and signal processing speed of the detector compared with a linear CCD, where signals are digitally added by an external circuit. Increased System Sensitivity In our spectrometers with linear CCDs, the slit’s width, not its height, regulates the amount of light entering the bench because linear CCDs cannot efficiently collect the light from the entire height of the slit. But in the QE65000, the 2D area detector can better take advantage of the height of the slit and the additional light, greatly improving system sensitivity. Excellent UV Response Because the detector in the QE65000 is back-thinned (or back-illuminated), it has great native response in the UV and does not require the additional coatings that we typically apply to other detectors for UV applications. Demanding Low Light-level Applications The QE65000 Spectrometer is a great option for low-light level applications including fluorescence, Raman spectroscopy, DNA sequencing, astronomy and thin-film reflectivity. The TE-cooled (down to -15 °C) detector features low noise and low dark signal, which enables low-light-level detection and long integration times (analogous to the shutter speed of a camera) from 8 milliseconds to 15 minutes, with virtually no spectral distortion. |
| An example of a fluorescence setup with a QE65000 uses the PX-2 Pulsed Xenon Lamp as an excitation source and the CUV-ALL Cuvette Holder for samples. An optical fiber delivers excitation light to the sample holder and read light to the spectrometer. A filter, such as one of our Linear Variable Filters would block excitation light from entering the spectrometer |
The QE65000 also has an onboard programmable micro-controller for controlling the spectrometer and accessories. You have access to 10 user-programmable digital inputs/outputs and a pulse generator for triggering other devices. You can use the I/Os to flash a lamp, stop/start a process, and send a message/alarm during the spectrometer’s integration period. The spectrometer’s operating parameters can be controlled through software. In fact, wavelength calibration coefficients unique to each spectrometer are programmed into a memory chip right on the spectrometer.
Optical Bench Options
At Ocean Optics, we understand that your work is unique and that you want a spectrometer to fit your specific needs. With the QE65000 Spectrometer, you can configure the bench for your application. Choose QE65000 Optical Bench Options such as entrance aperture size, detector accessories, filters, gratings and more. Our application scientists will help you determine which configuration will best work for you.
Variety of Filter Options Available
We have a number of variable bandpass order-sorting filter options available to vary the starting wavelength at 50 nm intervals to fit your particular application. These OFLVs are for use with the HC-1 grating only and consist of the following:
| OFLV Filter | Wavelength Range |
| OFLV-QE | 200-950 nm |
| OFLV-QE-250 | 250-1000 nm |
| OFLV-QE-300 | 300-1050 nm |
| OFLV-QE-350 | 350-1100 nm |
| OFLV-QE-400 | 400-1150 nm |
The QE65000 is available with external triggering that helps you easily synchronize data acquisition with external events. Check out the documentation (PDF format):
- External Triggering for Firmware versions below 3.0
- External Triggering for Firmware 3.0 and above
Our QE65000 Manual provides you with instructions for setting up, calibrating and performing experiments with your QE65000 spectrometer.
Specifications
| Physical | |
| Dimensions: | 182 mm x 110 mm x 47 mm |
| Weight: | 1.18 kg (without power supply) |
| Detector | |
| Detector: | Hamamatsu S7031-1006 |
| Detector range: | 200-1100 nm |
| Pixels: | 1024 x 58 (1044 x 64 total pixels) |
| Pixel size: | 24 μm2 |
| Pixel well depth: | 1000 Ke- |
| Sensitivity: | 22 electroncs/count all ٨; 26 photons/count at 250 nm |
| Quantum efficiency: | 90% peak; 65% at 250 nm |
| Optical Bench | |
| Design: | f/4, Symmetrical crossed Czerny-Turner |
| Focal length: | 101.6 mm input and output |
| Entrance aperture: | 5, 10, 25, 50, 100 or 200 µm wide slits or fiber (no slit) |
| Grating options: | Multiple grating options, UV through Shortwave NIR |
| HC1-QE grating option: | provides UV-Shortwave NIR coverage |
| Detector collection lens option: | None |
| OFLV filter options: | OFLV-QE (200-950 nm); OFLV-QE-250 (250-1000 nm); OFLV-QE-300 (300-1050 nm); OFLV-QE-350 (350-1100 nm); OFLV-QE-400 (400-1150 nm) |
| Other bench filter options: | Longpass OF-1 filters |
| Collimating and focusing mirrors: | Standard only |
| UV enhanced window: | No |
| Fiber optic connector: | SMA 905 to 0.22 numerical aperture single-strand optical fiber |
| Spectroscopic | |
| Wavelength range: | Grating dependent |
| Optical resolution: | ~0.14-7.7 nm FWHM |
| Signal-to-noise ratio: | 1000:1 (at full signal) |
| A/D resolution: | 16 bit |
| Dark noise: | 3 RMS counts |
| Dynamic range: | 7.5 x 109 (system), 25000:1 for a single acquisition |
| Integration time: | 8 ms to 15 minutes |
| Stray light: | <0.08% at 600 nm; 0.4% at 435 nm |
| Corrected linearity: | >99% |
| Electronics | |
| Power consumption: | 500 mA @ 5 VDC (no TE cooling); 3.5 A @ 5 VDC (with TE cooling) |
| Data transfer speed: | Full scans to memory every 8 ms with USB 2.0 port, 18 ms with USB1.1 port, 300 ms with serial port |
| Inputs/Outputs: | 10 onboard digital user-programmable GPIOs (general purpose inputs/outputs) |
| Analog channels: | No |
| Auto nulling: | Yes |
| Breakout box compatibility: | Yes, HR4-BREAKOUT |
| Trigger modes: | 4 modes |
| Strobe functions: | No |
| Gated delay feature: | Yes |
| Connector: | 30-pin connector |
| Power-up time: | <5 seconds |
| Dark current: | 4000 e-/pixel/sec @ 25 ºC; 200 e-/pixel/sec @ 0 ºC |
| Computer | |
| Operating systems: | Windows 98/Me/2000/XP, Mac OS X and Linux with USB port; Any 32-bit Windows OS with serial port |
| Computer interfaces: | USB 2.0 @ 480 Mbps; RS-232 (2-wire) @ 115.2 K baud |
| Peripheral interfaces: | SPI (3-wire); I2C inter-integrated circuit |
| Temperature and Thermoelectric (TE) Cooling | |
| Temperature limits: | 0 ºC to 50.0 ºC; no condensation |
| Set point: | Software controlled; lowest set point is 40 ºC below ambient |
| Stability | +/-0.1 ºC of set temperature in <2 minutes |
