描述
AmpliTaq? DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly charaerized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme:
AmpliTaq? DNA Polymerase is the most thoroughly charaerized enzyme available for the PCR process, a testimony to its overall utility and efficacy
Its thermal aivity profile makes it good for PCR applications
It is QC-tested to guarantee reproducible results
Good for PCRThe thermal aivity profile of AmpliTaq? DNA Polymerase is good for PCR applications because its optimal aivity is in the same range at which stringent annealing of primers occurs (55°C–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. AmpliTaq? DNA Polymerase is supplied with GeneAmp? 10X PCR Buffer I. It is also available with GeneAmp? 10X PCR Buffer II and MgCl
2 Solution.
Ultrapure optionAlso available is AmpliTaq? 360 DNA Polymerase for ior PCR results with the broadest range of templates. Compared to the original AmpliTaq? DNA Polymerase, AmpliTaq? 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating baerial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq? 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this produ is a 360 GC Enhancer to help work through challenging GC-rich templates.
For Research Use Only. Not for use in diagnostic procedures.
描述
?AmpliTaq DNA聚合酶是一种分子量为94 kDa，，重组DNA聚合酶的一种改进形式表达获得的水生栖热菌（Taq）在大肠杆菌DNA聚合酶基因。它是彻底的特点酶可用于聚合酶链反应过程中，其重组的性质和纯化方法提供的纯度和重现性，瓶到瓶，很多很多。
这种酶的特点：
?AmpliTaq DNA聚合酶是彻底的特点酶可用于PCR检测，证明其整体的实用性和性
它的热谱，使其的应用程序
它是质量控制测试，以可重复性的结果
和的聚合酶链反应
?的AmpliTaq DNA聚合酶的热谱是PCR应用好因为它的活动是在引物退火时严格相同的范围（55°C–75°C）。酶的半衰期是95°~ C 40分钟，提供的热稳定性，满足困难的PCR应用的要求。?AmpliTaq DNA聚合酶提供?03倍PCR缓冲液I也可与?03倍PCR缓冲液和氯化镁溶液。
规格
| Polymerase: | AmpliTaq? DNA Polymerase |
|---|---|
| Hot Start: | No |
| Fidelity (vs. Taq): | 1 X |
| Reaion Format: | Separate Components |
| Produ Overhang: | 3'-A |
| Reaion Speed: | Standard |
| Final Produ Size(s): | 5 kb or less |
| Produ Size: | 250 units |
| Exonuclease Aivity: | 5' - 3' |
| Shipping Condition: | Dry Ice |
内容及储存
Contains 250 units of AmpliTaq? DNA Polymerase and Buffer I. Store at –15 to –30°C.
